Comparison of Areas in the Mouth to Recover DNA Introduced Through Kissing
DOI:
https://doi.org/10.29173/jafn882Keywords:
DNA recovery, Oral swabs, Sexual assault, Oral sexAbstract
Sexual assault is a significant crime defined in the FBI’s Uniform Crime Report as “the penetration, no matter how slight, of the vagina or anus with any body part or object, or oral penetration by a sex organ of another person, without the consent of the victim” (FBI, 2017).
From January, 2019 to December, 2024, there were 537,446 reported rape victims in the United States (FBI, 2024). Oral-genital contact occurred between 18 and 25 percent of sexual assault cases (Marlia, 2011) (Sibille, 2002).
Contact may lead to foreign (male) DNA being deposited in the female mouth associating a perpetrator and victim. Recognizing that DNA does not survive indefinitely on human tissue, limits the time available to recover DNA for identification of the suspect from the victim.
Following a sexual assault many victims report to a healthcare facility for a comprehensive medical evaluation including evidence collection. Obtaining DNA evidence during this exam helps establish suspected sexual contact with the victim (Forrest, 2022).
Case prosecution with analyzed forensic evidence (including DNA) is known to increase conviction rates, but unless there is a scientific basis for the evidence to be admitted in Court under the Daubert Rule, evidence that links a perpetrator to a victim may be excluded.
Currently no scientifically based protocol exists for effective collection of suspect DNA from around and in the oral cavity of a victim. The purpose of this study is to provide empirically-based evidence to determine sensitive and probable areas to swab within the oral cavity.
References
Alderden, M., Cross, T. P., Vlajnic, M., & Siller, L. (2021). Prosecutors' perspectives on biological evidence and injury evidence in sexual assault cases. Journal of Interpersonal Violence, 36(7–8), 3880–3902. https://doi.org/10.1177/0886260518778259
Banaschak, S., Moller, K., & Pfeiffer, H. (1998). Potential DNA mixtures introduced through kissing. International Journal of Legal Medicine, 111(5), 284–285. https://doi.org/10.1007/s004140050172
Bernstein, D. E., & Jackson, J. D. (2004). The Daubert trilogy in the states. Jurimetrics Journal, 44, 351–336. https://www.jstor.org/stable/29762857
Bond, J. W., & Hammond, C. (2008). The value of DNA material recovered from crime scenes. Journal of Forensic Sciences, 53(4), 797–801. https://doi.org/10.1111/j.1556-4029.2008.00746.x
Brew-Graves, E., & Morgan, L. (2015). Injuries and allegations of oral rape: A retrospective review of patients presenting to a London sexual assault referral centre. Journal of Forensic and Legal Medicine. https://doi.org/10.1016/j.jflm.2015.06.004
Campbell, R., & Raja, S. (1999). Secondary victimization of rape victims: Insights from mental health professionals who treat survivors of violence. Violence and Victims, 14(3), 261–275. https://doi.org/10.1891/0886-6708.14.3.261
Centers for Disease Control and Prevention. (2025). Dental infection and control. https://www.cdc.gov/dental-infection-control/hcp/index.html
Cole, S. A., & Dioso-Villa, R. (2009). Investigating the “CSI effect”: Media and litigation crisis in criminal law. Stanford Law Review, 61(6), 1335–1374. https://heinonline.org/HOL/LandingPage?handle=hein.journals/stflr61&div=42&id=&page=
Cross, T. P., Siller, L., Vlajnic, M., & Alderden, M. (2022). The relationship of DNA evidence to prosecution outcomes in sexual assault cases. Violence Against Women, 28(15–16), 3910–3932. https://doi.org/10.1177/10778012221077124
Cross, T., & Alderden, M. (2018). Injury evidence, forensic evidence and the prosecution of sexual assault, United States, 2005–2011. Inter-university Consortium for Political and Social Research. https://doi.org/10.3886/ICPSR36608.v1
Department of Justice. (2016). National best practices for sexual assault kit. https://www.nsvrc.org/sites/default/files/2017-08/250384.pdf
Fahrney, P. M. (1975). Sexual assault package: A refinement of a previous idea. Journal of the American College of Emergency Physicians, 4(4), 340–341. https://doi.org/10.1016/S0361-1124(75)80089-X
Federal Bureau of Investigation. (2017). Crime in the United States. https://ucr.fbi.gov/crime-in-the-u.s/2017/crime-in-the-u.s.-2017/topic-pages/rape
Federal Bureau of Investigation. (2025). FBI uniform crime reporting program. https://cde.ucr.cjis.gov/LATEST/webapp/#/pages/explorer/crime/crime-trend
Fonneløp, A. E., Andersen, T. E., Bøstrand, L., & Egeland, T. (2019). A retrospective study on the transfer, persistence and recovery of sperm and epithelial cells in samples collected in sexual assault casework. Forensic Science International: Genetics, 43, 102153. https://doi.org/10.1016/j.fsigen.2019.102153
Forrest, S. (2022). Study examines impact of DNA evidence in sexual assault prosecutions. University of Illinois News Bureau. https://news.illinois.edu/study-examines-impact-of-dna-evidence-in-sexual-assault-prosecutions/
Gramlich, J. (2024). What the data says about crime in the U.S. Pew Research Center. https://www.pewresearch.org/short-reads/2024/04/24/what-the-data-says-about-crime-in-the-us/
International Association of Forensic Nurses. (2024). Sample policies and procedures for forensic nursing programs. https://www.safeta.org/wp-content/uploads/2024/03/FNPoliciesandProcedures.pdf
Johnson, D., Peterson, J., Sommers, I., & Baskin, D. (2012). Use of forensic science in investigating crimes of sexual violence: Contrasting its theoretical potential with empirical realities. Violence Against Women, 18(2), 193–222. https://doi.org/10.1177/1077801212440157
Jordan, J. (2024). Federal rules of evidence. The Committee on the Judiciary, House of Representatives. https://www.uscourts.gov/sites/default/files/2025-02/federal-rules-of-evidence-dec-1-2024_0.pdf
Kamodyova, N., Durdiakova, J., Celec, P., Sedlackova, T., Repiska, G., Sviezena, B., & Minarik, G. (2012). Prevalence and persistence of male DNA identified in mixed saliva samples after intense kissing. Forensic Science International: Genetics, 7(1), 124–128. https://doi.org/10.1016/j.fsigen.2012.07.007
Kell, G. (2017). August Vollmer biography explores famous police chief’s UC Berkeley ties. Berkeley News. https://news.berkeley.edu/2017/04/19/august-vollmer-biography-explores-famous-police-chiefs-uc-berkeley-ties/
Magalhães, T., Dinis-Oliveira, R. J., Silva, B., Corte-Real, F., & Vieira, D. N. (2015). Biological evidence management for DNA analysis in cases of sexual assault. The Scientific World Journal, 2015, 365674. https://doi.org/10.1155/2015/365674
Marlia, G. N. (2011). DNA yield of oral samples obtained after oral sex (master’s thesis, University of California, Davis). ProQuest Dissertations & Theses. (UMI No. 1502364)
McCall-Hosenfeld, J., Freund, K., & Liebschutz, J. (2009). Factors associated with sexual assault and time to presentation. Preventive Medicine, 48(6), 593–595. https://doi.org/10.1016/j.ypmed.2009.03.016
National Institute of Justice. (2017). National best practices for sexual assault kits: A multidisciplinary approach. https://nij.ojp.gov/topics/articles/national-best-practices-sexual-assault-kits-multidisciplinary-approach
National Research Council. (2009). Strengthening forensic science in the United States: A path forward. The National Academies Press. https://doi.org/10.17226/12589
Nittis, M., Franco, M., & Cochrane, C. (2016). New oral cut-off time limits in NSW. Journal of Forensic and Legal Medicine, 44, 10–13. https://doi.org/10.1016/j.jflm.2016.09.006
Office of Justice Programs. (1931). National Commission on Law Observance and Enforcement, Report No. 2. https://www.ojp.gov/pdffiles1/Digitization/44540NCJRS.pdf
Owers, R., McDonald, A., Montgomerie, H., & Morse, C. (2018). A casework study comparing success rates and expectations of detecting male DNA using two different Y-STR multiplexes on vaginal swabs in sexual assault investigations where no semen has been detected. Forensic Science International: Genetics, 37, 1-5. https://doi.org/10.1016/j.fsigen.2018.07.016
Peterson, J., & Sommers, I. (2010). The role and impact of forensic evidence in the criminal justice process (NIJ Grant Report No. 2006-DN-BX-K011). California State University, Los Angeles. https://www.ojp.gov/pdffiles1/nij/grants/231977.pdf
Pourhoseingholi, M. A., Mohsen, V., & Rahimzadeh, M. (2013). Sample size calculation in medical studies. Gastroenterology and Hepatology from Bed to Bench, 6(1), 14–17. https://pmc.ncbi.nlm.nih.gov/articles/PMC4017493/
Rape, Abuse & Incest National Network. (2025). Statistics. https://rainn.org/statistics
Roewer, L. (2019). Y-chromosome short tandem repeats in forensics—Sexing, profiling, and matching male DNA. WIREs Forensic Science, 1(4), e1336. https://doi.org/10.1002/wfs2.1336
Royal College of Pathologists Australia. (2022). Evidence based forensic sampling standards. https://www.rcpa.edu.au/getattachment/a83318be-18cf-42f9-9332-7856856131f1/Evidence-Based-Forensic-Sampling-Standards.aspx
Sibille, I., Duverneuil, C., de la Grandmaison, G. L., Guerrouache, K., Teissière, F., Durigon, M., & de Mazancourt, P. (2002). Y-STR DNA amplification as biological evidence in sexually assaulted female victims with no cytological detection of spermatozoa. Forensic Science International, 125(2–3), 212–216. https://doi.org/10.1016/S0379-0738(01)00650-8
Socscistatistics. (2025). Mann-Whitney test calculator. https://www.socscistatistics.com/tests/mannwhitney/default2.aspx
Speck, P., & Ballantyne, J. (2015). Detection of male DNA in the vaginal cavity after digital penetration using Y-chromosome short tandem repeats (Final Report, NIJ Grant No. 2009-DN-BX-0023).
StatKingdom. (2025). ANOVA test calculator. https://www.statskingdom.com/180Anova1way.html
Sween, K. R., Quarino, L. A., & Kishbaugh, J. M. (2015). Detection of male DNA in the vaginal cavity after digital penetration using Y-chromosome short tandem repeats. Journal of Forensic Nursing, 11(1), 33–40. https://doi.org/10.1097/JFN.0000000000000056
Sweet, D., & Shutler, G. G. (1999). Analysis of salivary DNA evidence from a bite mark on a body submerged in water. Journal of Forensic Sciences, 44(5), 1069–1072. https://doi.org/10.1520/JFS12045J
Tareck, A. (2024). Mouth anatomy. Medscape. https://emedicine.medscape.com/article/1899122-overview?form=fp
Thermo Fisher Scientific Inc. (2025). The AmpFLSTR Yfiler PCR amplification kit. https://www.thermofisher.com/order/catalog/product/4359513
Thermo Fisher Scientific. (2025). AmpFLSTR™ Yfiler™ PCR amplification kit user guide. https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0029852-AmpFLSTR-YFilerPCRAmpKit-UG.pdf
U.S. Department of Justice Office on Violence Against Women. (2024). A national protocol for sexual assault medical forensic examinations: Adults/adolescents (3rd ed.). https://www.justice.gov/ovw/media/1367191/dl?inline
Urbaniak, G. C., & Plous, S. (2025). Research randomizer. https://www.randomizer.org/#google_vignette
Williams, D., & Williams, J. (2019). The recovery of foreign DNA introduced through kissing. Podium session presented at the American Academy of Forensic Science Annual Meeting, Baltimore, MD.
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